Narishige Pc10 Manual New ((install)) Official

Determines the tip size and sharpness. 4. Step-by-Step Operating Procedures

Getting the perfect pipette often requires optimizing the parameters, especially if you are using new glass or a new machine.

Independent rotary knobs controlling the electrical current sent to the platinum coil during the first and second heating stages.

Tighten the lower clamp knob to secure the bottom of the glass tube. Ensure the glass is perfectly centered inside the loop of the heating coil without touching the metal filament. Step 2: The Filament Value Test (Finding the Melting Point) Set the Stage Mode toggle to . Turn the Heater Dial down to its lowest setting. Turn on the main Power Switch. Press the Start button.

The is a cornerstone in laboratory pipette fabrication, renowned for its ability to produce consistent, high-quality micropipettes for applications like patch clamping and microinjection . Although Narishige has transitioned to the PC-100 , the PC-10 remains a workhorse in many labs worldwide. narishige pc10 manual new

| Problem | Likely Cause | Fix | |--------|-------------|-----| | Glass melts but does not pull | Magnet power too low | Increase magnet power | | Glass breaks into pieces | Too much heat or too fast pull | Lower heater current or reduce weight | | Tip too long & floppy | Insufficient heat | Increase heater current or timer | | Pipette bent at tip | Uneven heating | Balance left/right heater currents | | No heating | Blown heater coil | Replace coil (order from Narishige) |

Understanding the physical layout and control interface is necessary before executing a pull. Physical Components

Tighten the terminal screws firmly. A loose connection will cause uneven heating and can damage the electronics. 3. Understanding the Control Panel

Understanding the physical layout is key to navigating the manual 1.2.1. Determines the tip size and sharpness

Understanding the anatomy of the PC-10 ensures precise adjustments and accurate troubleshooting. Core Component Checklist

The Narishige PC-10 isn’t just a puller; it’s a time capsule of mechanical genius. Before digital touchscreens and software updates, the PC-10 used gravity, a magnetic solenoid, and a carefully calibrated heater to pull glass capillaries into fine-tipped micropipettes. No firmware crashes. No DRM. Just physics and skill.

Without the original manual, new users often misunderstand the solenoid timing or incorrectly set the heater counterweight. This is why a new (legible, complete) manual is non-negotiable for safe and effective operation.

The PC-10 is compatible with various glass capillaries (e.g., borosilicate, aluminosilicate). Step 2: The Filament Value Test (Finding the

Mastering the Narishige PC-10: A Comprehensive Guide to the Dual-Stage Pipette Puller

Includes a transparent cover to shield the glass capillaries from air currents during the heating process. NARISHIGE WEB NEWS Typical Settings for Common Pipettes Settings vary by glass type, but general rules apply: NARISHIGE WEB NEWS High Heater Value: Produces long, thin pipettes. Low Heater Value: Produces short, thick pipettes. Double Pull:

Lower the heat settings; check for draft currents in the room. 5. Maintenance and Filament Replacement

Always wear safety glasses. Tensioned glass capillaries can shatter unexpectedly during the pulling sequence.